Protéus is pleased to announce the publication, in one of the editions of the prestigious journal Nature, of one of its enzyme test protocols developed in collaboration with Prof. Jean-Louis Reymond at the University of Bern, Switzerland.
In order to identify new enzymes by screening its unique collection of microorganisms or by using its proprietary methods for protein directed evolution, Protéus has developed new detection methods of biocatalytic activities. Among the enzymes targeted, hydrolases (including lipases/esterases, epoxide hydrolases, proteases, acylases…) represent a large family, widely used in industry. Simple, fast and versatile methods are therefore strongly required to rapidly identify enzymes suited for industrial needs.
The article describes one of these techniques, which also enables to define the catalytic fingerprint of these enzymes. Actually, the approach published enables to rapidly determine the activity pattern of the enzyme (i.e. its activity fingerprint) according to the type of substrate and the reaction conditions, which are key parameters for developing industrial biocatalysts.
Ref: Viviana S Fluxa, Denis Wahler and Jean-Louis Reymond
Enzyme assay and activity fingerprinting of hydrolases with the red-chromogenic adrenaline test
Nature Protocols 2008, 3(8), 1270-1277